Comparison of Extraction and Clean-up Methods Modified in Different Ways for the Determination of Pesticide Residues in Bees
Keywords:Bee, Pesticide, Residue, Extraction, Clean-up
As a result of the unconscious use of pesticides, honeybees are adversely affected, thus causing colony losses all over the world. In this research, different extraction and clean-up methods were compared on bee samples based on the QuEChERS method which is the most widely used pesticide analysis method in food. This research includes application studies for the multi residue method (MRM), which allows analysis of more than one pesticide. During the extraction phase, 7 different methods were compared, while the method with the most suitable repeatability and recovery values among these methods was taken to the clean-up phase. In the clean-up phase, 5 different clean-up methods were tried on the method which yielded the best values in extraction. In terms of average percentage recovery values, the 1st extraction method ranks first with 72 active substances between 70-120%, and the 3rd clean-up method with 92 active substances. Thus, while the number of active substances with suitable recovery values in the selected extraction method was 72, it increased to 92 with the continuation of the clean-up method, achieving a success rate of 92%. It is recommended to use this method in pesticide residue analyzes in bees with its outstanding features such as completing the analyzes in a short time, working easily with the infrastructure that can be found in all control laboratories, low analysis costs, ease of use, and reliability.
Akdogan, A., Divrikli, U., & Elci, L. (2012). Importance of pesticides and their effects on ecosystem. Academic Food Journal, 10(1), 125-132.
Alaux, C., Brunet, J.C., Dussaubat, C., Mondet, F., Tchamitchan, S., Cousin, M., Brillard, J., Baldy, A., Belzunces, L.P., & Le Conte, Y. (2010). Interactions between Nosema microspores and a neonicotinoid weaken honeybees (Apis mellifera). Environmental Microbiology, 12(3), 774-782. https://doi.org/10.1111/j.1462-2920.2009.02123.x
Bargańska, Ż., Konieczka, P., & Namieśnik, J. (2018). Comparison of two methods for the determination of selected pesticides in honey and honeybee samples. Molecules, 23(10), 2582. https://doi.org/10.3390/molecules23102582
Burdine, J.D., & McCluney, K.E. (2019). Differential sensitivity of bees to urbanization driven changes in body temperature and water content. Scientific Reports, 9:1643, 1-10. https://doi.org/10.1038/s41598-018-38338-0
EN 15662. (2018). European Standard, Foods of plant origin - Multimethod for the determination of pesticide residues using GC- and LC based analysis following acetonitrile extraction/partitioning and clean-up by dispersive SPE - Modular QuEChERS-method.
Karahan, A., Yıldırım, F., Karahan, M., & Karaca, İ. (2018). Does Imidacloprid Cause Winter Losses in Honey Bees? Arıcılık Araştırma Dergisi, 10(2), 54-60.
Karahan, A., Acar, İ., Kutlu, M.A., & Karaca, İ. (2019). Effect of Prohibited or Restricted Neonicotinoids on Bees in Turkey. 2nd International Agricultural Congress, Ankara, Turkey, 21-24 Nov 2019, p. 11-16.
Kasiotis, K.M., Anagnostopoulos, C., Anastasiadou, P., & Machera, K. (2014). Pesticide Residues in Honeybees, Honey and Bee Pollen By LC–MS/MS Screening: Reported Death İncidents in Honeybees. Science of the Total Environment, 485–486, 633–642. https://doi.org/10.1016/j.scitotenv.2014.03.042
Kiljanek, T., Niewiadowska, A., Semeniuk, S., Gawel, M., Borzecka, M., & Posyniak, A. (2016). Multi-residue method for the determination of pesticides and pesticide metabolites in honeybees by liquid and gas chromatography coupled with tandem mass spectrometry-Honeybee poisoning incident. J. Chromatogr. A, 1435, 100-114. https://doi.org/10.1016/j.chroma.2016.01.045
Li, Y., Wang, S., & Qin, Y. (2012). Supercooling Points of Apis mellifera ligustica when Performing Different Age-Related Tasks. Journal of Apicultural Science, 56 (1), 107-114. https://doi.org/10.2478/v10289-012-0012-z
Naggar, Y.A., Codling, G., Vogt, A., Naiem, E., Mona, M., Seif, A., & Giesy, J.P. (2015). Organophosphorus Insecticides in Honey, Pollen and Bees (Apis mellifera L.) and Their Potential Hazard to Bee Colonies in Egypt. Ecotoxicology and Environmental Safety, 114, 1–8. https://doi.org/10.1016/j.ecoenv.2014.12.039
Oruc, H.H., & Cayci, M. (2019). Honey Bee Deaths with Suspected Poisonings in Turkey. I. International VI. Veterinary Pharmacology and Toxicology Congress, Kayseri, Turkey, 4-7 Sep 2019, p. 161-170.
Polat, B., Ozuicli, M., Cetin, H., & Aydin, L. (2020). The Effects of Pesticide Usage on Honey Bee Health and Products. J Res Vet Med., 39(2), 128-134. https://doi.org/10.30782/jrvm.634586
Segmenoglu, M.S. (2020). The Effect of Pyrethroid Group Pesticides on Honey Bee Deaths in Çukurova Region. TURJAF, 8(3), 594-597. https://doi.org/10.24925/turjaf.v8i3.594-597.3025
Unal, H.H., Oruc, H.H., Sezgin, A., & Kabil, E. (2010). Determined Pesticides After Honey Bee Deaths Between 2006 and 2010 in Turkey. U. Bee J., 10(4), 119-125.
Vernich, P.C., Calatayud, F., Simó, E., & Picó, Y. (2016). Efficiency of QuEChERS approach for determining 52 pesticide residues in honey and honey bees. MethodsX, 3, 452-458. https://doi.org/10.1016/j.mex.2016.05.005
Wu, J.Y., Smart, M.D., Anelli, C.M., & Sheppard, W.S. (2012). Honey bees (Apis mellifera) reared in brood combs containing high levels of pesticide residues exhibit increased susceptibility to Nosema (Microsporidia) infection. Journal of Invertebrate Pathology, 109, 326-329. https://doi.org/10.1016/j.jip.2012.01.005
Yavuz, O., & Aksoy, A. (2016). The Methods Used in Pesticide Analysis. Türkiye Klinikleri J Vet Sci Pharmacol Toxicol-Special Topics. 2(2), 89-100.
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