The Studies on Seed Germination and in Vitro Cultures of Salvia L. Species from Turkish Flora

Authors

Keywords:

Salvia, Micropropagation, Ex-situ conservation

Abstract

Turkey has a rich flora in terms of plant biodiversity, and economically important Salvia species because of medicinal properties are in danger of extinction. To prevent the extinction of Salvia species is important to solve the problems of seed germination and micropropagation. In this review, in vitro studies conducted on Salvia species from Turkish flora were listed by specifying danger categories. Seed germination studies carried out 18 Salvia species [S. candidissima Vahl., S. cyanescens Boiss. & Balansa (endemic/LR-lc), S. dichroantha Stapf (endemic/LR-lc), S. fruticosa Mill. (VU), S. macrosiphon Boiss., S. microstegia Boiss. et Bal., S. nemorosa L., S. officinalis L., S. pomifera ssp. pomifera L. (VU), S. sclarea L., S. siirtica Kahraman, Celep & Doğan sp. nov. (endemic/CR), S. smyrnaea Boiss. (endemic/EN), S. tomentosa Mill., S. verbenaca L., S. verticillata L., S. virgata Jacq., S. viridis L.]. In vitro cultures studies were conducted on 16 Salvia species [S. aethiopis L., S. albimaculata Hedge and Huber-Morath (endemic/VU)-unsuccessful result, S. cadmica Boiss. (endemic/LR-lc), S. candidissima Vahl ssp. occidentalis Hedge, S. chrysophylla Stapf (endemic/LR-cd), S. cryptantha Montbret et Aucher ex Bentham (endemic/LR-lc), S. euphratica Montbret et Aucher ex. Bentham var. euphratica (endemic/LR-cd)-unsuccessful result, S. fruticosa (VU), S. nemorosa, S. nydeggeri Hub.-Mor.(endemic/EN)-unsuccessful result, S. officinalis, S. sclarea, S. tomentosa, S. verticillata ssp. verticillata, S. virgata, S. viridis]. In this review has been reported up to now in vitro cultures have been carried out 26 of 99 Salvia species, has clearly shows that new protocols should be established to protect endemic and threatened Salvia species.

References

Abdollahi, J., Ebrahimi, M., Ramshini, H. A., Jaafari, A. A., Eftekhari, M., Mansouri, Y. S., & Goharrizi, M. A. S. B. (2012a). Seed germination as the major conservation issue of endemic Iranian Salvia species. J. Med. Plant Res., 6(1), 37-46.

Abdollahi, J., Tafaroji, S. H., Ebrahimi, M., Ramshini, H. A., Tafti, A. R. D., Mortazavian, M., Eftekhari, M., Dezfoulian, A. H., & Bagheri, A (2012b). Effect of drought stress on germination and seedling growth of Salvia species. African Journal of Agricultural Research 7(43), 5719-5725. https://doi.org/10.5897/AJAR12.1954

Anonymous, (2007). National Biological Diversity Strategy and Action Plan. Ministry of Environment and Forestry. Republic of Turkey Ministry of Environment and Forestry.

Arikat, N. A., Jawaj, F. M., Karam, N. S., & Shibli, R. A. (2004). Micropropagation and accumulation of essential oils in wildsage (Salvia fruticosa Mill.). Sci. Hortic., 100, 193-202. https://doi.org/10.1016/j.scienta.2003.07.006

Arslan, D., Çığ, A., Arslan, H., Bayraktar, Ö. V., & Tülücü, F. (2017). Effects of treatments of gibberellic acid, citric acid and stratification on germination of seeds of Salvia siirtica Kahraman, Celep & Doğan sp. Nov. (Lamiaceae). J. Appl. Biol. Sci., 11(1), 29-32.

Aşkun, T., Başer, K. H. C., Tumen, G., & Kurkcuoğlu, M. (2010). Characterization of essential oils of some Salvia species and their antimycobacterial activities. Turk. J. Biol., 34, 89-95. https://doi.org/10.3906/biy-0809-2

Bürün, B. (2021). The use of biotechnology in conservation of plant biodiversity and studies in Turkey. Eskişehir Technical Univ. J. of Sci. and Tech. C- Life Sci and Biotech., 10(1), 1-16. https://doi.org/10.18036/estubtdc.590752

Çalışkan, T., Hatipoğlu, R., & Kırıcı, S. (2019). Production of plant secondary metabolites from cell and organ cultures under in vitro conditions. Turkish Journal of Agriculture - Food Science and Technology, 7(7), 971-980. https://doi.org/10.24925/turjaf.v7i7.971-980.2447

Dadaşoğlu, E., & Özer, H. (2014). Effects of different temperatures and gibberellic acid (GA3) doses on germination of Salvia species. Zeitschrift für Arznei- & Gewürzpflanzen, 19(1), 47-51.

Dastanpoor, N., Fahimi, H., Shariati, M., Davazdahemami, S., & Hashemi, S. M. M. (2013). Effects of hydropriming on seed germination and seedling growth in sage (Salvia officinalis L.) African Journal of Biotechnology, 12(11), 1223-1228. https://doi.org/10.5897/AJB12.1941

Demirci, T., Özdamar, P., & Göktürk-Baydar, N. (2015). In vitro applications for the increasing of root-related secondary metabolite production in medicinal plants and vegetables. Turkish Journal of Agriculture - Food Science and Technology, 3(5), 261-270.

Dowom, S. A., Abrishamchi, P., Radjabian, T., & Salami, S. A. (2017). Enhanced phenolic acids production in regenerated shoot cultures of Salvia virgata Jacq. after elicitation with Ag+ ions, methyl jasmonate and yeast extract. Industrial Crops and Products, 103, 81-88. https://doi.org/10.1016/j.indcrop.2017.03.043

Dumanoğlu, Z., & Mokhtarzadeh, S. (2020). Some physical properties of Salvia species seeds (Salvia hispanica L., Salvia tomentosa Mill. and Salvia verticillata L.) cultivated in Turkey. Turkish Journal of Agricultural and Natural Sciences, 7(3), 596-602. https://doi.org/10.30910/turkjans.663891

Durgha, H., Ramya, G., Ramanth, M. G., & Thirugnanasampandan, R. (2015). Standardization of medium for synthetic seed germination of Salvia sclarea L. Kong. Res. J. 2(1), 118-120. https://doi.org/10.26524/krj79

Elmas, S., & Elmas, O. (2021). Therapeutic use of Salvia fruticosa (Anatolian sage). International Journal of Life Sciences and Biotechnology, 4(1): 114-137. https://doi.org/10.38001/ijlsb.764602

Erişen, S., Kurt-Gür, G., & Servi, H. (2020). In vitro propagation of Salvia sclarea L. by meta-Topolin, and assessment of genetic stability and secondary metabolite profiling of micropropagated plants. Ind. Crops Prod., 157, 112892. https://doi.org/10.1016/j.indcrop.2020.112892

Ghanbar, T., Hosseini, B., & Jabbarzadeh, Z. (2012). Improving Salvia sclarea L. seed germination under in vitro condition. Intl. J. Agric: Res & Rev., 2(S), 1051-1058.

Ghanbar, T., Hosseini, B., Jabbarzadeh, Z., Farokhzadand, A., & Sharafi, A. (2016). High-frequency in vitro direct shoots regeneration from axillary nodal and shoot tip explants of clarysage (Salvia sclarea L.). Bulg. J. Agric. Sci., 22(1), 73-78.

Grzegorczyk, I., & Wysokińska, H. (2011). A protocol for synthetic seeds from Salvia officinalis L. shoot tips. Acta Biologica Cracoviensia Series Botanica, 53(1), 80-85. https://doi.org/10.2478/v10182-011-0011-6

Grzegorczyk-Karolak, I., Kuźma, Ł, Scała, E., & Kiss, A. (2018). Hairy root cultures of Salvia viridis L. for production of polyphenolic compounds. Industrial Crops and Products, 117, 235-244. https://doi.org/10.1016/j.indcrop.2018.03.014

Grzegorczyk-Karolak, I., Kuźma, Ł, Lisiecki, P., & Kiss, A. (2019). Accumulation of phenolic compound in different in vitro cultures of Salvia viridis L. and their antioxidant and antimicrobial potential. Phytochem. Lett., 30, 324-332. https://doi.org/10.1016/j.phytol.2019.02.016

Grzegorczyk-Karolak, I., Hnatuszko-Konka, K., Zarzycka, M., & Kuźma, Ł. (2020). The stimulatory effect of purine-type cytokinins on proliferation and polyphenolic compound accumulation in shoot culture of Salvia viridis. Biomolecules, 10(178), 1-15 https://doi.org/10.3390/BIOM10020178

Grzegorczyk-Karolak, I., Staniewska, P., Lebelt, L., & Piotrowska, D. G. (2021). Optimization of cultivation conditions of Salvia viridis L. shoots in the Plantform bioreactor to increase polyphenol production. Plant Cell, Tissue and Organ Culture (PCTOC), 1-13. https://doi.org/10.1007/s11240-021-02168-2

Ioja-Boldura, O. M., Radu, F., Popescu, S., & Borozan A. (2010). Regeneration, micropropagation, callus cultures and somatic embryogenesis of common sage (Salvia officinalis L.). Bulletin Uasvm Horticulture, 67(1), 308-3013.

İpek, A., & Gürbüz, B. (2010). Salvia species in flora of Turkey and their status in danger. Journal of Field Crops Central Research Institute, 19(1-2), 30-35.

Jafari, S., Daneshvar, M. H., Salmi, M. R. S., & Jalal-Abadi, A. L. (2017a). Influence of putrescine and thidiazuron on in vitro organogenesis in Salvia officinalis L. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 25(2), 200-211.

Jafari, S., Daneshvar, M. H., Salmi, M. R. S., & Jalal-Abadi, A. L. (2017b). Indirect organogenesis and plant regeneration in common Sage (Salvia officinalis L.): An important medicinal plant of Iran. Modern Applied Science, 11(5), 22-29. https://doi.org/10.5539/mas.v11n5p22

Jakovljević, M., Jokić, S., Molnar, M., Jašić, M., Babić, J., Jukić, H., & Banjari, I. (2019). Bioactive profile of various Salvia officinalis L. preparations. Plants, 8(55), 1-30. https://doi.org/10.3390/plants8030055

Javaid, M. M., Florentine, S., Ali, H. H., & Welle, S. (2018). Effect of environmental factors on the germination and emergence of Salvia verbenaca L. cultivars (verbenaca and vernalis): An invasive species in semi-arid and arid rangeland regions. Plos One, 13(3), 1-20. https://doi.org/10.1371/journal.pone.0194319

Joshi, S. C., & Pant, S. C. (2010). Physical purity analysis and germination of Salvia sclarea L. a medicinal plant. Nat. Sci., 8(1), 68-69. https://doi.org/10.7537/marsnsj080110.07

Kahraman, A., Doğan, M., & Celep, F. (2011). Salvia siirtica sp. nov. (Lamiaceae) from Turkey. Nord. J. Bot., 29, 397-401. https://doi.org/10.1111/j.1756-1051.2011.00916

Karam N. S., Jawad, F. M., Arikat N. A., & Shibli, R. A. (2003). Growth and rosmarinic acid accumulation in callus, cell suspension, and root cultures of wild Salvia fruticosa. Plant Cell Tissue Organ Cult., 73, 117-121. https://doi.org/10.1023/A:1022806420209

Kayıkçı, S., & Oğur, E. (2020). A Study on detection of rare and endemic Salvia L. species in Hatay province. Anadolu, J. of Aari, 30(2), 197-206. https://doi.org/10.18615/anadolu.834912

Khakpor, A., Bibalani, G. H., & Mahdavi, K. (2011). Influence of scabbing and hot water treatment on germination of sage plant (Salvia verticillata) seeds. Ann. Biol. Res., 2(5), 52-55.

Khawar, K. M., Ünver, T., & Özcan, S. (2003). In vitro induction of crown galls by Agrobacterium tumefaciens super virulent strain A281 (pTiBo542) in Salvia sclarea and S. pratense. Biotechnol. Biotechnol. Equip., 17(2), 63-69. https://doi.org/10.1080/13102818.2003.10817060

Kintzios, S., Nikolaou, A., & Skoula, M. (1999). Somatic embryogenesis and in vitro rosmarinic acid accumulation in Salvia officinalis and S. fruticosa leaf callus cultures. Plant Cell Rep., 18, 462-466. https://doi.org/10.1007/s002990050604

Kivrak, S., Göktürk, T., Kivrak, I., Kaya, E., & Karababa, E. (2019). Investigation of phenolic profiles and antioxidant activities of some Salvia species commonly grown in Southwest Anatolia using UPLC-ESI-MS/MS. Food Sci. Technol, Campinas, 39(2), 423-431. https://doi.org/10.1590/fst.32017

Kumar, R., & Sharma, S. (2012). Effect of light and temperature on seed germination of important medicinal and aromatic plants in north western Himalayas. Int. J. Med. Arom. Plants, 2(3), 468-475.

Kümmritz, S., Haas, C., Pavlov, A. I., Geib, D., Ulber, R., Bleya T., & Steingroewer, J. (2014). Determination of triterpenic acids and screening for valuable secondary metabolites in Salvia sp. suspension cultures. Natural Product Communications, 9(1), 17-20.

Liu, W., Chilcott, C. E., Reichand, R. C., & Hellmann, G. M. (2000). Regeneration of Salvia sclarea via organogenesis. In Vitro Cell. Dev. Biol.-Plant, 36, 201-206.

Lopresti, A. L. (2017). Salvia (Sage): A review of its potential cognitive-enhancing and protective effects. Drugs, 17, 53-64. https://doi.org/10.1007/s40268-016-0157-5

Mascarello, C., Mantovani, E., & Ruffoni, B. (2006). In vitro culture of several ornamental and medicinal Salvia species. ISHS Acta Hortic., 723: I International Symposium on the Labiatae: Advances in Production, Biotechnology and Utilisation. https://doi.org/10.17660/ActaHortic.2006.723.51

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiology Plantarum, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Nitsch, J. P., & Nitsch, C. (1969). Haploid Plants from Pollen Grains. Science, 163, 85-87. http://dx.doi.org/10.1126/science.163.3862.85

Öğütçü, H., Sökmen, A., Sökmen, M., Polissiou, M., Serkedjieva, J., Daferera, D., Şahin, F., Bariş, Ö., & Güllüce, M. (2008). Bioactivities of the various extracts and essential oils of Salvia limbata C.A.Mey. and Salvia sclarea L. Turk. J. Biol., 32, 181-192.

Özcan, I. I., Arabacı, O., & Öğretmen, N.G. (2014). The determination of different germination applications on some sage species. Turkish Journal of Agriculture - Food Science and Technology, 2(5), 203-207. https://doi.org/10.24925/turjaf.v2i5.203-207.58

Petrova, M., Nikolova, M., Dimitrova, L., & Zayova, E. (2015). Micropropagation and evaluation of flavonoid content and antioxidant activity of Salvia officinalis L. Genetics and Plant Physiology, 5(1): 48-60.

Rezaeieh, K. A. P., Shidfar, M., Gürbüz, B., & Khavar, K. M. (2012). Genomic DNA extraction from seed induced callus and explants in Salvia L. species for utilization in secondary metabolite production. Journal of Medicinal Plants Research 6(4), 636-640. https://doi.org/10.5897/JMPR11.1382

Saruhan-Fidan, H., Kilinc, F. M., Yilmaz, M. A, Akdeniz, M., Yener, I., Firat, M., Onay, A., Kolak, U., & Ertas, A. (2021). Comparison of chemical and biological properties of in vivo and in vitro samples of Salvia siirtica Kahraman, Celep & Dogan extracts prepared with different solvents. South African Journal of Botany, 142, 421-429. https://doi.org/10.1016/j.sajb.2021.07.029 0254-62

Sharma, Y., Fagan, J., & Schaefer, J. (2019). Ethnobotany, phytochemistry, cultivation and medicinal properties of garden sage (Salvia officinalis L.). Journal of Pharmacognosy and Phytochemistry, 8(3), 3139-3148.

Skała, E., & Wysokińska, H. (2004). In vitro regeneration of Salvia nemorosa L. from shoot tips and leaf explants. In Vitro Cell. Dev. Biol. Plant, 40, 596-602. https://doi.org/10.1079/IVP2004580

Sönmez, Ç., Gökçöl, A., Şimşek Soysal, A. Ö., Bayram, E., & Çelen, A. E. (2019). Research on germination and emergence performance enhancing treatments on sage (Salvia spp.) species. Turkish Journal of Agriculture - Food Science and Technology, 7(3), 504-510. https://doi.org/10.24925/turjaf.v7i3.504-510.2318

Subaşı, Ü., & Güvensen, A. (2010). Seed germination studies on rare endemic Salvia smyrnaea Boiss. (Lamiaceae). Bio. Di. Con., 3(3), 126-132.

Surgun Acar, Y., & Bürün, B. (2017, May 10-12). Plant tissue culture studies in genus Salvia and its importance. I. International Congres on Medicinal and Aromatic Plants ‘Natural and Healthy Life’.

Thanos, C. A., & Doussi, M. A. (1995). Ecophysiology of seed germination in endemic labiates of Crete. Isr. J. Plant Sci., 43, 227-237. https://doi.org/10.1080/07929978.1995.10676607

Tosun, M., Ercisli, S., Sengul, M., Ozer, H., Polat, T., & Ozturk, E. (2009). Antioxidant properties and total phenolic content of eight Salvia species from Turkey. Biol. Res., 42, 175-181. http://dx.doi.org/10.4067/S0716-97602009000200005

Tursun, A. Ö. (2019). The effects of different applications on breaking dormancy of Salvia verticillata L. (Lilac Sage). KSU J. Agric. Nat., 22(Suppl 1), 30-37. https://doi.org/10.18016/ksutarimdoga.vi.560605

Tursun, A. Ö. (2020). The effects of low temperature applications on dormancy of Salvia verticillata L. and Rumex crispus L. seeds. Pak. J. Bot., 52(6), 1885-1890. https://doi.org/10.30848/PJB2020-6(18)

Ustun, O., Ozcelik, B., & Baykal, T. (2016). Bioactivities of ethanolic extract and its fractions of Cistus laurifolius L. (Cistaceae) and Salvia wiedemannii Boiss. (Lamiaceae) species. Pharmacognosy Magazine, 12(45), 582-585. https://doi.org/10.4103/0973-1296.176125

Uyanık, M. (2017). In vitro propagation of some endemic Salvia species which are endangered in Turkey and adaptation to field conditions. [Doctoral dissertation, Ankara University].

Uysal, S., Zengin, G., Sinan, K. I., Ak, G., Ceylan, R., Mahomoodally, M. F., Uysal, A., Sadeer, N. B., Jek, J., Czi, Z., Rodrigues, M. J., Yıldıztugay, E., Elbasanh, F., & Custodiog, L. (2021). Chemical characterization, cytotoxic, antioxidant, antimicrobial, and enzyme inhibitory effects of different extracts from one sage (Salvia ceratophylla L.) from Turkey: open a new window on industrial purposes. RSC Adv., 11, 5295-5310.

Vulganová, K., Maliar, T., Maliarová, M., Nemeček, P., Viskupičová, J., Balážová, A., & Sokol, J. (2019). Biologically valuable components, antioxidant activity and proteinase inhibition activity of leaf and callus extracts of Salvia sp. Nova. Biotechnol. Chim., 18(1), 25-36.

Yaschurevskaya, M.N., & Cherednichenko, M.Y. (2017, July 05-08). In vitro seed sterilization of some Salvia species and varieties. The 3rd International Symposium on EuroAsian Biodiversity, Minsk, Belarus.

Yilar, M., Kadioglu, I., & Telci, I. (2018). Chemical composition and antifungal activity of Salvia officinalis (L.), S. cryptantha (Montbret Et Aucher Ex Benth.), S. tomentosa (Mill.) plant essential oils and extracts. Fresenius Environmental Bulletin 27(3), 1695-1706.

Yücel, E., & Yilmaz, G. (2009). Effects of different alkaline metal salts (NaCl, KNO3), acid concentrations (H2SO4) and growth regulator (GA3) on the germination of S. cyanescens Boiss. & Bal. seeds. G.U. Journal of Science, 22(3), 123-127.

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2022-06-15

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Yilmaz Gokdogan, E., & Burun, B. (2022). The Studies on Seed Germination and in Vitro Cultures of Salvia L. Species from Turkish Flora. Natural Products and Biotechnology, 2(1), 60–73. Retrieved from https://natprobiotech.com/index.php/natprobiotech/article/view/25

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Review Article