Chemical Composition and Antibacterial Activity of Helichrysum italicum (Roth) G. Don Commercial Essential Oil from Serbia



Helichrysum italicum, Essential oil, Antibacterial activity


Medicinal plants have been preferred sources against drug-resistant pathogens recently. The antimicrobial activities of essential oils have been known for a long time. In the present research, the chemical composition and antibacterial activity of commercial essential oil of Helichrysum italicum (Roth) G. Don (HIE) were evaluated. Seventy components which constitute 95.7%, were identified in the oil. The major constituents of HIE were neryl acetate (15.6%), α-pinene (11.5%), and γ-curcumene (11.3%). The oil was screened for antibacterial activity by agar well diffusion and broth microdilution methods. The essential oil displayed remarkable antibacterial activity against test bacteria, except for Escherichia coli, which displayed resistance. The minimum inhibitory concentration (MIC) of essential oil were 5 µg/mL for Staphylococcus aureus and Pseudomonas aeruginosa, 10 µg/mL for Bacillus cereus and B. subtilis. These results suggested that the commercial oil of H. italicum has the potential to be used as a natural antibacterial agent.


Aćimović, M., Ljujić, J., Vulić, J., Zheljazkov, V. D., Pezo, L., Varga, A., & Tumbas Šaponjac, V. (2021). Helichrysum italicum (Roth) G. Don Essential Oil from Serbia: Chemical Composition, Classification and Biological Activity—May It Be a Suitable New Crop for Serbia?. Agronomy, 11(7), 1282.

Andreani, S., Uehara, A., Blagojević, P., Radulović, N., Muselli, A., & Baldovini, N. (2019). Key odorants of industrially-produced Helichrysum italicum subsp. italicum essential oil. Industrial Crops and Products, 132, 275-282.

Angioni, A., Barra, A., Arlorio, M., Coisson, J. D., Russo, M. T., Pirisi, F. M., & Cabras, P. (2003). Chemical composition, plant genetic differences, and antifungal activity of the essential oil of Helichrysum italicum G. Don ssp. microphyllum (Willd) Nym. Journal of Agricultural and Food Chemistry, 51(4), 1030-1034.

Appendino, G., Ottino, M., Marquez, N., Bianchi, F., Giana, A., Ballero, M., & Munoz, E. (2007). Arzanol, an anti-inflammatory and anti-HIV 1 phloroglucinol α-pyrone from Helichrysum italicum ssp. microphyllum. Journal of Natural Products, 70(4), 608-612.

Azaz, A. D., Kürkcüoglu, M., Satil, F., Can Baser, K. H., & Tümen, G. (2005). In vitro antimicrobial activity and chemical composition of some Satureja essential oils. Flavour and Fragrance Journal, 20(6), 587-591.

Bianchini, A., Tomi, P., Costa, J., & Bernardini, A. F. (2001). Composition of Helichrysum italicum (Roth) G. Don fil. subsp. italicum essential oils from Corsica (France). Flavour and Fragrance Journal, 16(1), 30-34.<30::AID-FFJ941>3.0.CO;2-F

Blažeković, N., Petričić, J., Stanić, G., & Maleš, Ž. (1995). Variations in yields and composition of immortelle (Helichrysum italicum, Roth Guss.) essential oil from different locations and vegetation periods along Adriatic coast. Acta Pharmaceutica, 45(4), 517-522.

Ćavar Zeljković, S., Šolić, M. E., & Maksimović, M. (2015). Volatiles of Helichrysum italicum (Roth) G. Don from Croatia. Natural Product Research, 29(19), 1874-1877.

Chauhan, R.S., Kitchlu, S., Ram, G., Kaul, M.K., & Tava, A. (2010). Chemical composition of capillene chemotype of Artemisia dracunculus L. from North-West Himalaya, India. Industrial Crops and Products, 31(3), 546-549.

Chinou, I. B., Roussis, V., Perdetzoglou, D., & Loukis, A. (1996). Chemical and biological studies on two Helichrysum species of Greek origin. Planta Medica, 62(04), 377-379.

Chopra, I. (2007). The increasing use of silver-based products as antimicrobial agents: a useful development or a cause for concern?. Journal of Antimicrobial Chemotherapy, 59(4), 587-590.

Djihane, B., Wafa, N., Elkhamssa, S., Maria, A. E., & Mihoub, Z. M. (2017). Chemical constituents of Helichrysum italicum (Roth) G. Don essential oil and their antimicrobial activity against Gram-positive and Gram-negative bacteria, filamentous fungi and Candida albicans. Saudi Pharmaceutical Journal, 25(5), 780-787.

Fraternale, D., Flamini, G., & Ascrizzi, R. (2019). In Vitro anticollagenase and antielastase activities of essential oil of Helichrysum italicum subsp. italicum (Roth) G. Don. Journal of Medicinal Food, 22(10), 1041-1046.

Gismondi, A., Di Marco, G., & Canini, A. (2020). Helichrysum italicum (Roth) G. Don essential oil: Composition and potential antineoplastic effect. South African Journal of Botany, 133, 222-226.

Mastelic, J., Politeo, O., Jerkovic, I., & Radosevic, N. (2005). Composition and antimicrobial activity of Helichrysum italicum essential oil and its terpene and terpenoid fractions. Chemistry of Natural Compounds, 41(1), 35-40.

Melito, S., Petretto, G. L., Podani, J., Foddai, M., Maldini, M., Chessa, M., & Pintore, G. (2016). Altitude and climate influence Helichrysum italicum subsp. microphyllum essential oils composition. Industrial Crops and Products, 80, 242-250.

Mollova, S., Fidan, H., Antonova, D., Bozhilov, D., Stanev, S., Kostova, I., & Stoyanova, A. (2020). Chemical composition and antimicrobial and antioxidant activity of Helichrysum italicum (Roth) G. Don subspecies essential oils. Turkish Journal of Agriculture and Forestry, 44(4), 371-378.

Nostro, A., Bisignano, G., Cannatelli, M. A., Crisafi, G., Germano, M. P., & Alonzo, V. (2001). Effects of Helichrysum italicum extract on growth and enzymatic activity of Staphylococcus aureus. International journal of Antimicrobial Agents, 17(6), 517-520.

Nostro, A., Cannatelli, M. A., Marino, A., Picerno, I., Pizzimenti, F. C., Scoglio, M. E., & Spataro, P. (2003). Evaluation of antiherpesvirus-1 and genotoxic activities of Helichrysum italicum extract. The New Microbiologica, 26(1), 125-128.

Oliva, A., Garzoli, S., Sabatino, M., Tadić, V., Costantini, S., Ragno, R., & Božović, M. (2020). Chemical composition and antimicrobial activity of essential oil of Helichrysum italicum (Roth) G. Don fil.(Asteraceae) from Montenegro. Natural Product Research, 34(3), 445-448.

Paolini, J., Desjobert, J. M., Costa, J., Bernardini, A. F., Castellini, C. B., Cioni, P. L., & Morelli, I. (2006). Composition of essential oils of Helichrysum italicum (Roth) G. Don fil subsp. italicum from Tuscan archipelago islands. Flavour and Fragrance Journal, 21(5), 805-808.

Rosa, A., Deiana, M., Atzeri, A., Corona, G., Incani, A., Melis, M. P., & Dessì, M. A. (2007). Evaluation of the antioxidant and cytotoxic activity of arzanol, a prenylated α-pyrone–phloroglucinol etherodimer from Helichrysum italicum subsp. microphyllum. Chemico Biological Interactions, 165(2), 117-126.

Sala, A., Recio, M. D. C., Giner, R. M., Máñez, S., Tournier, H., Schinella, G., & Ríos, J. L. (2002). Anti-inflammatory and antioxidant properties of Helichrysum italicum. Journal of Pharmacy and Pharmacology, 54(3), 365-371.

Šćepanović, A., Krivokapić, S., Šćepanovic, V., Živković, V., & Perović, S. (2019). Chemical constituents and biological potential of essential oils of Helichrysum italicum (Roth) G. Don from Montenegro. Poljoprivreda i Sumarstvo, 65(2), 53-58.

Servi, H., Şen, A., Servi, E. Y., & Doğan, A. (2021). Chemical composition and biological activities of essential oils of Foeniculum vulgare Mill. and Daucus carota L. growing wild in Turkey. Journal of Research in Pharmacy, 25(2), 142-152.

Staver, M. M., Gobin, I., Ratkaj, I., Petrovic, M., Vulinovic, A., Dinarina-Sablic, M., & Broznic, D. (2018). In vitro antiproliferative and antimicrobial activity of the essential oil from the flowers and leaves of Helichrysum italicum (Roth) G. Don growing in central Dalmatia (Croatia). Journal of Essential Oil Bearing Plants, 21(1), 77-91.

Stupar, M., Ljaljević-Grbić, M., Džamić, A., Unković, N., Ristić, M., & Vukojević, J. (2014). Antifungal activity of Helichrysum italicum (Roth) G. Don (Asteraceae) essential oil against fungi isolated from cultural heritage objects. Archives of Biological Sciences, 66(4), 1539-1545.

Tzanova, M., Grozeva, N., Gerdzhikova, M., Atanasov, V., Terzieva, S., & Prodanova, R. (2018). Biochemical composition of essential oil of Corsican Helichrysum italicum (Roth) G. Don, introduced and cultivated in South Bulgaria. Bulgarian Journal of Agricultural Science, 24, 1071-1077.

Vieira, T. M., Dias, H. J., Medeiros, T. C., Grundmann, C. O., Groppo, M., Heleno, V. C., & Silva, E. O. (2017). Chemical composition and antimicrobial activity of the essential oil of Artemisia absinthium Asteraceae leaves. Journal of Essential Oil Bearing Plants, 20(1), 123 131.




How to Cite

Yildirim Servi, E., & Servi, H. (2022). Chemical Composition and Antibacterial Activity of Helichrysum italicum (Roth) G. Don Commercial Essential Oil from Serbia. Natural Products and Biotechnology, 2(1), 14–22. Retrieved from



Research Article