Genome Wide Analysis and Characterization of NPR-like Gene Family of Phaseolus vulgaris L.

Burak Muhammed  Oner; Emre Ilhan; Ayse Gul  Kasapoglu; Selman Muslu; Ahmed Sidar  Aygoren; Sumeyra  Ucar; Esra Yaprak; Murat  Isıyel; Recep Aydinyurt; Murat Aydin

Authors

Burak Muhammed Oner Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey https://orcid.org/0000-0003-2785-2089
Emre Ilhan Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey https://orcid.org/0000-0002-8404-7900
Ayse Gul Kasapoglu Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey https://orcid.org/0000-0002-6447-4921
Selman Muslu Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey https://orcid.org/0000-0002-2084-760X
Ahmed Sidar Aygoren Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey https://orcid.org/0000-0002-6264-9935
Sumeyra Ucar Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey https://orcid.org/0000-0002-7629-0206
Esra Yaprak Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey https://orcid.org/0000-0002-8753-494X
Murat Isıyel Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey https://orcid.org/0000-0003-4157-2729
Recep Aydinyurt Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey https://orcid.org/0000-0003-3743-1835
Murat Aydin Department of Agricultural Biotechnology, Faculty of Agriculture, Atatürk University, Erzurum, Turkey https://orcid.org/0000-0003-1091-0609

Keywords:

NPR1, SAR, Stress, In silico, Phaseolus vulgaris L.

Abstract

Nonexpressor of pathogenesis-related genes 1 (NPR1) could be found in the regulation of pathways of salicylic acid (SA) signal transduction and systemic acquired resistance (SAR) in plants. It has been found that the members of NPR-like gene family are interconnecting with biotic and abiotic stresses in several plants, but it has not yet been performed in common bean. In this study, in silico analyzes of Phaseolus vulgaris L. NPR-like gene family members were performed at the genome level. As a result of these in silico analyzes, four NPR1 genes were identified and it was found that these genes have been located on four different P. vulgaris chromosomes. The length of PvNPR-like proteins ranges from 475-590 amino acid residues. The theoretical isoelectric point (pI) range were found between 5.75-6.36 and all show acidic properties. As a result of phylogenetic analyzes performed between NPR-like genes of P. vulgaris, Glycine max (L.) Merr and Arabidopsis thaliana (L.) Heynh. plants, NPR-like genes were found to be divided into three different groups (A, B and C). It has been determined that PvNPR-like genes contain cis-acting elements that function in many biological processes, including stress and hormone responses. Differences in gene expression levels under salt and drought stress indicated that PvNPR-like genes respond differently under stress conditions. The outcomes of this study will supply the needed basics for understanding the function and evolutionary history of PvNPR-like genes.

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Genome Wide Analysis and Characterization of NPR-like Gene Family of Phaseolus vulgaris L.

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