Nepeta italica L. subsp. cadmea (Boiss.) A. L. Budantsev Oil Has a Pro-Apoptotic Effect on Saos-2 Cell Line

Siddika Boztas; Buket Er Urganci; Mucahit Secme; Nazan Keskin

Authors

Siddika Boztas Histology and Embriology Department, Faculty of Medicine, Pamukkale University, Denizli, Turkey https://orcid.org/0000-0001-9315-2174
Buket Er Urganci Medical Biology Department, Faculty of Medicine, Pamukkale University, Denizli, Turkey https://orcid.org/0000-0002-5339-3835
Mucahit Secme Medical Biology Department, Faculty of Medicine, Ordu University, Ordu, Turkey https://orcid.org/0000-0002-2084-760X
Nazan Keskin Histology and Embriology Department, Faculty of Medicine, Pamukkale University, Denizli, Turkey https://orcid.org/0000-0002-5488-9322

Keywords:

Nepeta, Saos-2, Apoptosis, Osteosarcoma

Abstract

Nepeta sp. are used diuretic, anti-spasmodic, antiasthmatic, expectorant, cough suppressant, sedative, antiseptic, antipyretic, antiallergy and as a muscle relaxant. Besides, it also has many biological activities such as antioxidant, antibacterial, antiinflammatory, anticancer, antiparasitic, antiplatelet, antiangiogenesis, antimicrobial, antiviral, antifungal, insecticide, cytotoxic, phytotoxic, genotoxic, and apoptotic activities. Osteosarcoma is the most common primary bone cancer in children and adolescents, and its diagnosis is difficult due to the known heterogeneous nature of its pathogenesis. Chemotherapy for osteosarcoma is far from satisfactory, especially because of the presence of metastases or poor response to combination chemotherapy. Various studies have been conducted to evaluate the anti-apoptotic, anticancer, and cytotoxic potentials of different essential oil extracts from different species of the Nepeta. This study was carried out to confirm the cytotoxic and apoptotic effect of essential oil obtained by hydrodistillation from the aerial parts (flower, leaf, seed and stem) of Nepeta italica subsp cadmea. To figure out apoptotic properties of N. italica subsp cadmea. MTT assay, qRT-PCR with Bcl‐2, caspase-9, Caspase-3, caspase-8, caspase-7, caspase-10, Bax gene primer and TUNEL assay accomplished. IC₅₀dose of N. italica subsp. cadmea oil was 10 μg/ml at 24 hours. Expression analysis showed that caspase8, caspase 10 were upregulated while Bcl-2 was downregulated. TUNEL assay results show the similar number of apoptotic cells in the control group then the Nepeta group. Therefore, it showed pro-apoptotic activity in conjunction with the expression profile.

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