Conservation in Tissue Culture of Malacocarpus crithmifolius (Retz.) Fisch. & C.A.Mey. - Relict Species from Mangyshlak

Valentina Mursaliyeva; Balaussa  Sarsenbek; Tlek Mukhanov; Akjunus Imanbayeva

Authors

Valentina Mursaliyeva Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan https://orcid.org/0000-0003-4803-9169
Balaussa Sarsenbek Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan https://orcid.org/0000-0002-7951-4586
Tlek Mukhanov Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan https://orcid.org/0000-0002-7590-769X
Akjunus Imanbayeva Mangyshlak Experimental Botanical Garden, Aktau, Kazakhstan https://orcid.org/0000-0003-0101-6840

Keywords:

Malacocarpus crithmifolius, Relict species, Regeneration, Micropropagation, Conservation

Abstract

Malacocarpus crithmifolius is an endangered rare relict with small size populations grown in a limited area. The purpose of this study was to initiate in vitro and micro replication of Malacocarpus crithmifolius. The vegetative and reproductive explants and Murashige & Skoog medium (MS) supplemented with different concentrations and combinations of cytokinin and auxins were used. The response reaction of different explants and the plant growth regulators' effects on shooting frequency, the number of shoots, callusogenesis, and rooting percent were investigated. Culturing generative explants on the MS medium containing 6-Benzylaminopurine (BAP) combined with indole-3-acetic acid (IAA) or indole-butyric acid (IBA) led to direct shoot regeneration. The bud mass appeared in the leaf axil under inflorescences after one week of cultivation on the initiated medium. The replication was reached by micro-cutting of induced additional shoots and 3-fold passaging them on the fresh mediums. The average multiplication result at MS with BAP in combination with 1-Naphthaleneacetic acid (NAA) or IAA composed 5.85 after three months of cultivation. In conclusion, the applicable explant and phytohormonal composition of nutrient medium for initiation and micropropagation of M. crithmifolius were developed.

References

Arzumanov, V.A., Butkov, E.A., Turdiev, M.K., Baymetov, K.I., & Yushev, A.A. (2015). Plant resources of fruit and the nut-fruited crop of Central Asia and their role in the formation of a local assortment. Bakttria Press, Uzbekistan, pp. 93-94.

Baytulin, I.O. (2010). Theoretical base and methodological approaches to the plant introduction in the region with extreme climatical conditions. Bulletin of NAS RK. Series biol. and medical, 2: 18-26.

Baytulin, I.O., Sitpaeva, G.T. (Eds.) (2014). The Red Book of Kazakhstan. Plants. Vol. 2, part 1., 2th ed., ArtPrintXX1, Astana, p. 452.

Belousova, L.C., Denisova, L.V., & Nikitina, C.V. (1979). Rare plants of USSA. Forestry industry Press, Moscow, p. 201.

Dzhanalieva, K.M., Budnikova, T.I., & Veselov, E.N. (1998). Physical geography of the Republic of Kazakhstan. Kazak University, Almaty.

Gemedjieva, N., Jaime, A., Teixeira da, S., & Ryabushkina, N. (2010). Representation of endemic in floristic sub-provinces of Kazakhstan. Asian Australas J Plant Sci Biotechnol., 4 (1): 56-63.

Imanbayeva, A., Belozerov, I., Kosareva, O., Kulakova, K., Dosshieva, G., & Lesnichenko, V. (2017). Collections of plants of Mangyshlak Botanical Garden. Luxe Media Publishing, Almaty.

Fay, M.F. (1992). Conservation of Rare and Endangered Plants Using in vitro Methods. In Vitro Cell. Dev. Biol. – Plant, 28P (1), 1–4.

Johnson, K.A. (2002). In vitro conservation including rare and endangered plants, heritage plants and important agricultural plants. In: Taji A. and Williams R. (Eds.) The importance of plant tissue culture and biotechnology in plant sciences. University of New England Publications Unit, Australia, p. 79-90.

Murashige, T., & Skoog, E. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum, 15: 473-497.

Pavlov N.V. (Ed.) (1963). Flora of Kazakhstan. Vol:6. Nauka. Alma-Ata, p. 32-33.

Ryabushkina, N., Gemedjieva, N., Kobaisy M., & Cantrell, C. (2008). Brief review of Kazakhstan flora and use of its wild species. Asian Australas J Plant Sci Biotechnol., 2(2): 64-71.

Shomurodov, H.F., Saribayeva,Sh. U., & Akhmedov, A. (2015). Distribution pattern and modern status of rare plant species on the Ustyurt plateau in Uzbekistan. Arid Ecosyst., 5(4): 261-267. https://doi.org/10.1134/S2079096115040125

Sokolov P.D. (Ed.). (1988). Plant resources of USSA. Flowering plants, its chemical composition, application. Families Rutaceae-Elangnaceae. Nauka, Leningrad. Vol.4, p. 33.

Takhtadzhian, A.L., Crovello, Th. J., & Cronquist, A. (1986). Floristic regions of the world. University of California Press, Berkeley.

Zharekeev, V.H., Telezhenetskaya, M.V., & Yunusov, Y.U. (1971). An investigation of the alkaloids of Malacocarpus crithmifolius. Chem. Nat. Comp., 7(4): 524. https://doi.org/10.1007/BF00564776